Docking Studies of Piperine-Vitamin a Conjugate to Study the Increase in Bioavailability of Vitamin A

Piperine, a major constituent of Piper nigrum (Black pepper), is one of the well known components in many Ayurvedic formulations. Piperine is most studied bioenhancer because it inhibits drug metabolizing enzymes in rodents and increases plasma concentrations of several drugs, including P-glycoprotein substrates. However, there are no evidences on piperine-vitamin A conjugate to inhibit human CYP450 3A4. In this paper the influence of piperine-vitamin A conjugate to study the metabolism of vitamin A with CYP450 3A4 was investigated. In silico results showed in the present study when piperine was conjugated with vitamin A, CYP450 3A4 was inhibited. This method improved the binding of piperine-vitamin A conjugate with CYP450 3A4 and increased bioavailability of Vitamin A.