Application of Response Surface Methodology in Process Parameter Optimization of Media for Production of Amylase

Agricultural wastes are being discarded and those wastes get accumulated in the environment. On the other hand, amylase production has become significant for its use in various industries like food, fermentation, brewing, detergent and paper industries. For this elevating demand, amylase production has to be increased. By using the agricultural waste as substrate source amylase enzyme can be produced in higher quantities. Using Bacillus subtilis as microbial source, agricultural waste can be utilised for the production of amylase in submerged fermentation as well as in solid state fermentation. Usage of synthetic media is very expensive and hence normal nutrient broth can be used. By using OVAT procedure, parameters such as temperature, pH, and substrate concentration can be varied and the optimum level at which enzyme production is higher can be determined. Response surface methodology is used for efficient production of enzyme. The enzyme produced in this process could be useful in reducing the demand of amylase in commercial use.