HPTLC Fingerprinting Analysis of Tannin Profile on Canthium coromandelicum and Flueggea leucopyrus willd.

The study of chemical composition of medicinal plants or drugs derived from plants is said to be Phytochemistry. Tannins are polyphenolic compounds; they are identified as better antiseptic due to the presence of phenolic group. The main objective of this study was to progress the High Performance Thin Layer Chromatography (HPTLC) fingerprinting of tannin profile on methanolic extracts of leaves and stem of Canthium coromandelicum and Flueggea leucopyrus willd aerial parts. The HPTLC fingerprinting analysis was carried for tannin using CAMAG LINOMAT 5 instrument which revealed that the methanolic extracts of Canthium coromandelicum leaves, stem and aerial parts Flueggea leucopyrus showed the presence of 10, 8 and 7 peaks with Rf values in the range of 0.04 to 0.97 respectively. Thus, the HPTLC methods developed in this study against the methanolic extract of selected plants provide useful and simple tools for the quality assessment and identification of phytochemical marker compounds. By isolating and identifying the biomarker compounds, new drugs can be synthesised to treat various ailments.