New stability indicating RP-UFLC method for the simultaneous determination of Velpatasvir and Sofosbuvir in tablets

A new liquid chromatographic method has been developed for the simultaneous determination of Sofosbuvir and Velpatasvir in oral dosage forms. Shimadzu Model CBM-20A/20 Alite with C8 (Phenomenex) column (250 mm × 4.6 mm i.d., 5 μm particle size) was used for the chromatographic study. Linearity was observed over the concentration range 4.54–400.14 μg/ml and 1.09–100.36 μg/ml with regression equations y = 26855x + 83874 (correlation coefficient 0.9997) and y = 35840x + 8543.8 (correlation coefficient 0.9999) for Sofosbuvir and Velpatasvir respectively. Sofosbuvir and Velpatasvir were subjected to forced degradation and the method was validated as per ICH guidelines.