IL-10-1082A\G gene polymorphism and production in β-thalassemia major and association with HCV infection

Thalassemia is the most common congenital hemolytic anemiadue to partial or complete lack of synthesis of _globin chains caused by mutations that affect the synthesis this-chains. The aim of this study is the detection of IL-10-1082A\G polymorphism and IL-10 serum level with it correlation to pathogeneses of disease. Casecontrol study was performed of 60 patients with β-TM diagnosed at thalassemia-center in AL-Zahra hospital in AL-Najaf city, Iraq with group of 40 healthy individual was used as control, the patients were (26 male and 34 female) at age3-49 and about 18 patients infected with virus. Blood sample and serum was collected from all patients and control. Blood used for DNA extracted for using SSP-PCR in detection the IL-10-1082A\G polymorphism. IL-10 level were measured by enzyme-linked immunosorbent assay test. The result shown that male (56.6%) more than female (42.6%), and the age range (10–19) were highest than other age range. This result explain that HCV infected patients less than non-infected thalassemia patients and the infected male more than female, the age group (10–19) was more infected with HCV. The result demonstrate that AA genotype and A allele is risk factor of severity in thalassemia patients, while GG genotype and G allele is protective factor for severity. The result explain that GG genotype is risk factor for HCV infection. This result also shown that IL-10 level is significantly increase in thalassemia patients than control, also significantly increase in IL-10 level in thalassemia with HCV infection than other patients with no HCV infection and control.

The polymorphism in IL-10 at position (1082A\G) has association with pathogeneses of thalassemia at AA genotype, and IL-10 consider as predictive factor for severity of thalassemia