Diagnosis and Distinguishing of Entamoeba spp. by PCR Technique

This study conducted between October 2016 to September 2017, with the aims of distinguishing between amoeba species by molecular methods and determination of the prevalence of pathogenic and non-pathogenic species, In Imam Hussein Teaching Hospital, in the province of ThiQar southern Iraq. 96 stool samples were positive in microscopic examination. After the PCR test, positive samples were found in 86 samples and 10 negative samples E.histolytica were the most common 82 (85.4%) Followed by E.dispar14 (14.5%) These two parasites mixed infection E.histolytica+E.dispar 10 (10.4%) cases, and E.histolytica+ E. hartmanni 8 (8.3%) cases.