Pro-inflammatory Cytokine Production Inhibitory Effects of Everlasting Oil

This study aims to demonstrate pro-inflammatory cytokine production inhibitory effects using everlasting oil.

Thus, This paper was carried out to see secretion of NO, inflammatory cytokine,TNF-α, IL-1β using the macrophages of LPS-treated mice. The cytotoxicity test was used 96 AQueous One solution cell proliferation assay. No Detection kit was used to measure No,the production of pro-inflammatory cytokine was measured using ELISA kit.

As a result, everlasting oil had no cytotoxicity at 5 ug/mL to 500 ug/mL and significantly inhibited the production of NO as well as TNF-α, IL-1β. In the RAW 264.7 mousemacrpphage cells,Pro-inflammatory cytokine production inhibitory effects are likely to be diversely utilized as basic physiological activity data and functional materials to demonstrate antiinflammatory properties of everlasting oil.

In conclusion, this study can be used to a basic data to objectively demonstrate the physiological activity of immunological mechanism associated with the anti-inflammatory action of everlasting oil, but it is necessary to conduct studies on anti-inflammation.