Determination of Gallic Acid in Ayurvedic Polyherbal Formulation Triphala churna and its ingredients by HPLC and HPTLC

Triphala churna is well known Ayurvedic formulation in official Ayurvedic Formulary of India, It is used for immune system stimulation, improvement of digestion, relief of constipation, gastrointestinal tract cleansing, relief of gas, treatment of diabetes, eye disease, anaemia, jaundice, asthma, fever, chronic ulcers etc. Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of the drug. In this, study attempts to evaluate the qualitative estimation of bio active compounds Gallic acid and Ellagic acid by HPTLC and quantification of Gallic acid by HPTLC and HPLC Chromatographic methods. The method employed in current study resulted in good peak shape of Gallic acid. The Rf value of Ellagic acid 0.47 and Gallic acid is 0.56 in all the samples and reference standard was found comparable under UV light at 254 nm and 366 nm. The percentage of free Gallic acid is prominent in Emblica officinalis and lower in Terminalia chebula of Triphala churna ingredients.