Development of the Technique of Quantitative Determination of Antracene derivatives in roots of Rheum officinale

This paper discusses the current aspects of improvement of methods of standardization of roots of Rheum officinale. The new approaches are proposed for the quantitative analysis of the content of anthracene derivatives of roots of Rheum officinale, which consists in the using of the method of spectrophotometry at analytical wavelength of 510 nm calculated on Frangula-emodin and the use of ultrasound for the maximum yield of anthracene derivatives of raw materials and reducing the time of extraction in a water bath. The existing method for the determination of anthracene derivatives in the roots of Rheum palmatum presented in the State Pharmacopoeia of the Russian Federation, XIV edition, provides for a long extraction in a water bath. The use of physical factors, such as ultrasound, increases the yield of biologically active substances in the extraction due to the destruction of the cell structure, which reduces the extraction time.