Determination of Flucloxacillin Sodium by Analytical Spectrophotometry

Simple and rapid spectrophotometric method has been developed for the determination of Flucloxacillin sodium (FLU) in raw material. The method is based on the formation of yellow ion-pair complex between Flucloxacillin sodium and Bromocresol purple (BCP) in Chloroform medium. The absorption maximum of the complex was found to be 408 nm. Different parameters affecting the reaction were optimized such as: effect of solvents, time, reagent concentration, correlation ratio, etc. The formed complex was quantified spectrophotometrically at absorption maximum. Linearity range was 2.47 – coefficient R² = 0.9999. The limit of detection (LOD) and limit and 0.615 μg/mL. The method was successfully applied for the determination of Flucloxacillin sodium in raw material. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.