Development of Analytical method by RP-HPLC Technique for determination of Secnidazole in API and Pharmaceutical Dosage Form

A novel reverse phase liquid chromatographic method protozoal, anti-amebic and antigiardiasis drug, secnidazole in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out on isocratic system. The column was Peerless basic C18 (50mm x 4.6mm, 3μm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 80:20% (v/v). The flow rate was maintained at 1 ml/min. The detection was carried out at wavelength 310 nm. validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50–150 μg/ml for secnidazole. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination secnidazole of in dosage form with good recoveries.