1Department of Pharmaceutics, KMCH College of Pharmacy, Coimbatore-641048
2Department of Pharmaceutics, Parul University, Gujarat
Enzymes are proteins that catalyze chemical reactions. The manufacture of an enzyme for use as a drug is an important facet of today's pharmaceutical industry. Microbial L-Asparaginase is widely used as a therapeutic agent in the treatment of certain human cancers, mainly in acute lymphoblastic leukemia. Fungal isolate was isolated from soil samples and it was collected from different regions of the Arabian Sea, using potato dextrose agar (PDA) medium by serial dilution method. The thermophilic fungus was isolated from that isolates and twelve isolates were selected and the isolated strain was screened by plate assay method using Czepk Dox medium and potential strain was used for the production of L-Asparaginase. From this work we conclude that more than 80% of the fungal strains from marine soil sample had the ability to produce the enzyme L-Asparaginase. High productivity of L-asparaginase was optimized at 30˚C in pH 7at 48hours of incubation period with 1ml of inoculam size and 200rpm/min of agitation. Important parameters like purification, immobilization (silica gel and agarose) and effect of activator (magnesium chloride) and inhibitor (EDTA) also evaluated. The highest immobilization yield 45.24% was obtained with Silica gel carrier.
Enzymes, Marine soil, L-Asparaginase, Penicillium notatum, Thermophilic fungi
