1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, India
2Shantha Biotechnics, Hyderabad, Telangana, India
With the increase market of the transgenic crops, the demand for testing Bt cotton seeds has increased dramatically. ELISA abased protein detection and PCR based gene identification are still major approaches for detecting GMOs on large scale.
In our present study, PCR and ELISA based techniques was adopted to identify the specific Cry1Ac gene and proteins in MECH 162 and RCH2 transgenic cotton plants.
MECH 162 of 21.9ug/g and RCH2 of 17.37ug/g of seed protein was extracted. PCR analysis confirmed that gene specific CRY1Ac F/R primer as amplicon size of 550 bp for Bt gene, 600bp for nptII gene and 200bp for 35s promoter gene.
The ELISA method was used for the validation of the developed assay, also ELISA needed simple equipment and took less time.
MECH 162, RCH2, GMO, Cry1Ac, NptII, 35s Promoter
