Antioxidant and Hepatoprotective activity of Ehretia laevis Roxb against paracetamol induced acute Hepatotoxicityin wistar rats

The aim of the current study is to evaluate the antioxidant activity and hepatoprotective activity of hydroalocoholic (70%) extract and ethyl acetate fraction of Ehretia laevis Roxb in wistar rats against paracetamol-induced acute hepatotoxicity. In addition, reducing the power of the extracts and their ability to scavenge free radicals were evaluated by applying 2, 2-diphenyl 2-picrylhydrazyl hydrate (DPPH) and Reducing Power Assay. The hydroalocoholic (70%) extract and its ethyl acetate fraction of Ehretia laevis displayed strong antioxidant activity. The extracts at a dose of 100 and 200 mg/kg, p.o., showed significant dose-dependent protection against paracetamol-induced changes in the serum ASAT, ALAT, ALP, and TP. The hydroalocoholic (70%) extract and its ethyl acetate fraction also showed dose-dependent protection against Paracetamol-induced changes in liver tissue such as fatty degeneration, lymphatic infiltration, and necrosis. Among the extracts tested the ethyl acetate fraction shows better activity than the Hydroalocoholic extract.