Detection of IgE, Some Antioxidant Parameter Levels and their Association with Polymorphism of ADAM33 Gene in Asthma Patients

Asthma is a multifactorial disease influenced by interactions between genetic and environmental factors. The alterations in some immunological, genetic and antioxidant markers can affecting pathogenesis of asthma, therefore, this study aimed to assessment of a disintegrin and metalloproteinase 33 V4 G/C (ADAM33 V4 C/G) polymorphism and its associated immunoglobulin E (IgE) antibodies and some antioxidant parameters with asthma in some Iraqi asthmatic patients. Blood samples collected from seventy asthmatic patients and forty healthy subjects (males and females) aged (18–70 year). Enzyme linked immunosorbent assay has been used for serum IgE estimation and other antioxidant parameters were carry out using biochemical methods for both groups. Genomic DNA was extracted from all blood samples and detected of ADAM33 V4 C/G polymorphism using Polymerase Chain Reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results showed that: there was a significant increase in IgE concentration (p <0.01) while antioxidant levels (glutathione, total Protein and albumin) decreased (p>0.01) in asthmatic patients compared with controls. there was a highly significant differences in alleles and genotypes frequency between patient and healthy group (p< 0.0001), frequency of G allele and genotype GG were higher in asthmatic patients compared with controls and there was a correlation between high IgE level and the presence of allele G within the patient genotypes. We can conclude that concentration of IgE and frequency of GG genotype were elevated in asthmatic patients compared with healthy. GG genotype and allele G can be considered as a genetic marker of the disease and presence of this allele is associated with the high levels of IgE and increase of disease severity.