Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity

Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low costeffective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.