Influence of Organic acids (Acetic, Citric acid and Blend) In vitro on growth of E. coli O157: H7: in Poultry feed

The study carried out at the possibility of employ organic acids with chicken feed to inhibits microorganism porn in feed, that which causes deteriorate and corruption of this feed, as well as the transmission of diseases, which are a threat to public health. Ten samples were prepared from 0.25 g of poultry feed and sterilized with autoclave and then infected with E. coli O157: H7 bacteria then incubated for two hours. Organic acids were added to the poultry feed in different percentage of acetic acid, citric acid and blind (0.5, 1, and 1.5) and incubated for 24 hours. Bacterial count was performed for each sample through 60 min. The influence of the acid concentration was noted when compared between the different groups, the blend acids B3 (1.5%) groups (3.88±0.041) showed high significant effect (P > 0.05) than acetic and citric acid group (A, C) groups at 60 minutes of time. The available growth time of E. coli O157H7 that contaminated chicken feed acetic, citric acid and both (blend) of them (0.5, 1, 1.5) % on the growth of E. coli were showed exhibited tolerance for 120 minutes except the blend group (B3) 1.5%, was showed no growth (0.00) in all group after 180 minutes of incubation.