1Department of Biology, Collage of Science, Mustansiriyah University, Iraq
2Directorate of Education in Babylon, Ministry of Education, Iraq
4Faculty of Health and Medical Sciences, Medical Technical University, Baghdad, Iraq
Online published on 24 December, 2019.
Coagulase-negative staphylococci (CNS) exemplify common skin commensals. They are opportunistic microorganisms. It has been recently confirmed that CNS can produce superantigens of Staphylococcus aureus. Different clinical samples (251) were collected from Al-Musayyib General Hospital in Babylon, Iraq. Sixty isolates were CNS, identified by conventional methods and Vitek2 system. The isolates were Staphylococcussciuri (31.66%), Staphylococcus haemolyticus (25%), Staphylococcussaprophyticus (23.33%) and Staphylococcus lentus (20%), the isolate obtained from seminal fluid, blood, tonsil and wound infections. All isolates showed resistant to methicillin antibiotic. Multiplex PCR was used to detect the presence of mecA, sea, seb, seg, seh, sei, selpand tst1 in Methicillin resistant CNS. This study suggested that MRCNS isolates from patient carried sea, seb, seg, seh, sei, and tst1superantigenic toxin genes.
CNS.mecA. S. haemolyticus, S. lentus. S. saprophyticus. S. sciuri. Superantigen
