Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2020
  • Volume: 13
  • Issue: 1

Carbohydrates determination in the Snowdrops (Galanthus L.) herbal pharmaceutical substances by TLC and UV-Spectrophotometry

  • Author:
  • D.O. Bokov1,2, I.R. Kulaeva3, O.G. Potanina4, E.V. Sergunova1, A.A. Bondar1, A.A. Evgrafov1, A.M. Antsyshkina1, I.I. Krasnyuk1
  • Total Page Count: 7
  • Page Number: 243 to 249

1Institute of Pharmacy, Sechenov First Moscow State Medical University, 8 Trubetskaya St., bldg. 2, Moscow, 119991, Russian Federation

2Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14 Ustyinsky pr., Moscow, 109240, Russian Federation

3Medical Institute, Chechen state University, 32 Sheripova St., Grozny, Chechen Republic, 364061, Russian Federation

4Pharmaceutical chemistry and pharmacognosy chair, Peoples’ Friendship University of Russia (RUDN University), 6, Miklukho-Maklaya Street, Moscow, 117198, Russian Federation

Online published on 24 February, 2020.

Abstract

Plants of Galanthus L. genus (snowdrops) used in Russian medicine for obtaining neurological and cardiovascular remedies, contain different types of carbohydrates (polysaccharides, monosaccharides) as additional biological active substances group. In present research the carbohydrate composition and content were investigated in the snowdrops herbal pharmaceutical substances (Galanthus woronowii Losinsk, Galanthus nivalis L.). Optimal conditions for TLC analysis, the extraction of crude herbal drugs, were determined; spectrophotometric procedures for quantifying the total carbohydrate content in terms of monosaccharides (fructose, glucose) were successfully developed. The results of the investigation can be used in quality control and standardization of crude herbal drugs containing carbohydrates, and for inclusion in pharmacopoeial monographs.

Keywords

carbohydrates, Galanthus woronowii Losinsk, Galanthus nivalis L, herbal pharmaceutical substances, glucose, fructose