1Bharath Institute of Higher Education and Research, Selaiyur, Chennai-600073, India
2SMS Pharmaceuticals Ltd, R&D Centre (A DSIR approved), Gagillapur, Telangana-500047, India, svr.org@gmail.com
A simple, Accurate, precise method was developed for the simultaneous determination of the Ivabradine and Metoprolol in Pharmaceutical dosage form. Chromatogram was run through SB C8 100 x 3.0mm, 1.8μm. Mobile phase containing Buffer 0.01N Potassium dihydrogen ortho phosphate: Acetonitrile chosen in the ratio 50: 50v/v was pumped through column at a flow rate of 0.3 ml/min. Temperature and Wave length was optimized at 30°C and 260 nm independently. Percentage of recovery was obtained as 100.03% and 100.03% for Ivabradine and Metoprolol individually. LOD, LOQ values obtained from regression equations of Ivabradine and Metoprolol were 0.03, 0.08 and 0.12, 0.35 respectively.
New method development, Determination, Ivabradine, Metoprolol, RP-UPLC
