HPTLC and antioxidant activity of ethanolic extract of Ipomoea obscura (L.) ker gawl stem

The aim was to optimize the extraction method and reveal the phytoconstituents using chromatographic detection and antioxidant activity from the stem part of Ipomoea obscura using DPPH assy.

The extraction was carried out using maceration process. The TLC parameters were set by trial and error method and suitable mobile phase for the constituent. The phytoconstituents from ethanolic extract were identified at 365nm by HPTLC. Additionally antioxidant activity is evaluated using UV/Visible spectrophotometer.

The % yield of extract was found as 39.37%w/w. From the phytochemical test, the metabolites such as alkaloids, carbohydrates, phenolic compound such as flavonoid were present. The metabolic product of the stem was separated using respective mobile phase through R_f value. Among these, only phenolic compound were choosed for HPTLC detection.

The mentioned HPTLC profile for the plant of interest will hopefully help the researchers in herbal field to develop more herbal medicines requiring the particular constituent or its extract.