Immunomodulatory activity of crude bromelain of Pineapple (Ananas comosus (L.) merr.) crown from Subang district, Indonesia

Crude bromelain, which extracted from pineapple (Ananas comosus (L.) Merr) crown from Subang district, West Java, Indonesia, has been shown to have protease and antioxidant activity. Both of these activities can be applied as immunomodulator agent. The purpose of this study was to determine the immunomodulatory activity of crude bromelain of the pineapple crown through phagocytic response and specific humoral immune response. The phagocytic response was observed from carbon clearance in induced-mice with black ink, while the specific humoral immune response was observed from antibody haemagglutination of mice which challenged with sheep red blood cells. Data were analyzed with one-way ANOVA and compared to the standard. The results showed that the immunomodulatory activity of crude bromelain was dose-independent in 1.56, 3.12, and 4.68 mg/20 g BW of mice. It was concluded that crude bromelain 3.12 mg/20 g BW has immunomodolatory activity comparable to the standard.