Protective effects of gossypin in colchicine-induced cognitive dysfunction and oxidative damage in rats

Gossypin is known to have antioxidant property and its neuroprotective activity in the ischemia model has been reported. There are no reports on the activity of Gossypin in the learning and memory. The objective of the current work was to evaluate the protective effects of Gossypin in Colchicine-induced Cognitive Dysfunction in rats. For this experiment Male Wistar rats were used and were administered with intracerebroventricular injection of colchicine and oral solution of gossypin (10 or 20 mg/kg) for 3 week to determine the preventive effect of gossypin against colchicine toxicity. Exposure to colchicine produced oxidative stress in rats and model for deficit in memory. The activity of Gossypin in enhancing memory was evaluated using Morris Water Maze for parameters related to spatial memory as Escape latency, island entries, etc. followed by biochemical estimation of AChE enzyme level in the frontal cortex and Hippocampal region of rat brain. Docking interaction study for understanding affinity of Gossypin to AChE enzyme was done using Schrodinger suite. Insilico XP docking study suggested the strong affinity of Gossypin to AChE. Treatment with Gossypin showed significant reduction of the AChE level in frontal cortex but not in Hippocampal region. Gossypin treated group showed slight reduction but not significant in the latency period. However significant increase in the number of island entries was observed. Overall, Gossypin showed significant in silico interaction with AChE, increased AChE activity and slight cognition enhancing property.