A study on marine bacterial invertase enzyme sourced from bay of Bengal, West Bengal; Its isolation and optimization

In this study, we have focused on isolation and characterization of invertase enzyme produced from marine bacteria. Marine bacteria have proved to have excellent potential for producing varied novel natural compounds and nanoparticles that are having a wide range of applications. In the current study, a total of 42 bacterial isolates were found from marine water collected from Bakkhali, West Bengal. A total of 20 Selected isolates were kept for UV-mutation and 15 UV-mutated marine bacterial isolates were screened for invertase enzyme activity. Out of them, bacterial isolate SPAB3 showed highest enzyme activity. For the 3 potential isolates, optimization was done for the production of the invertase enzyme in different environmental conditions. The optimum conditions for the potential isolate SPAB2 were galactose as carbon source, 37°C and pH 7. For SPAB3, the optimum conditions were glucose as carbon source, 20°C and pH 7. For SPAB4, the optimum conditions were mannitol as carbon source, 20°C and pH 7. The potential isolates SPAB2, SPAB3 and SPAB4 were characterized using macroscopic, microscopic and biochemical tests and identified as Bacillus sp., Staphylococcus sp. and Staphylococcus sp. respectively.