Genetic study of txo a gene in Pseudomonas aeruginosa isolated from burn wound infections

The bacterium Pseudomonas aeruginosa is a chief causative agent of hospital infections, and contamination with antibiotic-resistant strains may be dangerous. A total of 35 wound burn specimens were collected from patients, and 15 isolates were recognized as P. aeruginosa by microbial cultures and VITIK. Bacterial genomic DNA was extracted, and the presence of the tox A gene was detected by PCR by using specific primers. The results revealed that toxA gene was prevalent in all studied local strains (100 %) from burn wound infections. In the current study most of the mutations in toxA sequence were trans version and transition. Studying and understanding of virulence genes in P. aeruginosa may facilitate the development of novel vaccines and drug treatments in the future.