Development and validation of simultaneous estimation of metformin hydrochloride and glimepiride in physiological media

Metformin and Glimepiride combinations are recently developed by various researches and Pharmaceutical industry. Hence it is needs to develop simple, precise, accurate, reliable and cost-effective method for Simultaneous determination of Metformin and Glimepiride. As per Pharmacopoeial recommendation pH 6.8 phosphate buffer is suitable dissolution method for combination of Metformin Hydrochloride and Glimepiride. In this study UV method is developed for simultaneous determination of Metformin Hydrochloride and Glimepiride. This involves measurement of absorption maxima at wavelength of about 226 nm and 233 nm for Glimepiride and Metformin Hydrochloride. In this study development and validation as per ICH Q2 (R1) is carried out. Various parameters such as Specificity, Solution Stability, Filter Compatibility, Filter Saturation, Linearity and Range, Precision, Accuracy were evaluated for validating developed method for determination of content of Metformin and Glimepiride.