Effect of Allium sativum, Curcuma mangga and Acorus calamus combination on the uterus and hormonal profile in rat induced by cisplatin

This research aimed to find out whether the combination of Allium sativum, Curcuma mangga, Acorus calamus extracts affect the weight of the uterus, uterus histology and estrogen-progesterone profile of cisplatin-induced rats. This experimental consist of seven treatments and four replications: untreated rats (K-), cisplatin 5mg/Kg BW (K+), dose extract of 50mg/Kg BW (P1), 75mg/Kg BW (P2), 100mg/Kg BW (P3), subur kandungan herbs (P4) and clomiphene citrate 0.9mg/Kg BW (P5). Uterine weight was obtained through weighing. Uterine histological slide were observed by the light microscope, whereas hormonal profile employed by ELISA method. The data were analyzed using the Anova continued with honest significant difference test. The result revealed that cisplatin induction significantly decreased the number of endometrial glands. The administration of extract combination significantly affected the uterine weight, endometrial thickness, number of endometrial glands and estrogen levels of cisplatin-induced rats. The dose of 75mg/Kg BW had the potential to increase the weight of the uterus, endometrial thickness and number of endometrial glands.