Recent quantitative analysis of stevioside from Stevia rebaudiana (Bertoni): A review

Stevia is a sweet-taste shrub that containing secondary metabolites called steviol glycoside. The main steviol glycoside in Stevia rebaudiana Bertoni is stevioside that 200-300 times sweeter than sucrose, which is contained in large amounts. This compound has benefited as a low-calorie sweetener and some biological activities. Therefore, the use of stevioside in various fields is increasingly widespread. The increasing use of stevioside from Stevia rebaudiana Bertoni on various products makes the analysis of this compound become important. Sensitive analysis methods are needed for the quantification of stevioside. Various methods have been developed for quantitative analysis of stevioside that produce various values of precision, accuracy, and sensitivity. This paper aims to summarize and describe the current literature on quantitative methods for stevioside from Stevia rebaudiana Bertoni. So that a comparison can be made between several methods to get the most suitable method for quantitative analysis. In addition, this paper also looks at the extent to which the success of the existed methods at the last decade assessed from various validation parameters so that they can be considered in the development of further analysis methods.