The properties of hydrolysed collagen from Oreochromis mossambicus's scale and their effect towards Cell viability

The purpose of this study was to determine the properties of hydrolysed collagen from Orechromis mossambicus's scales. The properties determination includes pH measurement, macronutrient evaluation, and molecular weight determination. Meanwhile, the effect of hydrolysed collagen at various concentrations on the viability of normal skin fibroblast primary cells has also examined. The pH value of this hydrolysed collagen was in the vicinity of 5. The moisture content, ash content, fat content, and protein content showed 3.65%, 0.79%, 0.22% and 63.1%, respectively. The polypeptide profile obtained in the SDS-PAGE analysis was between 17.75 to 23.50 kDa. Besides, 4mg/mL until 10mg/mL of hydrolysed collagen concentrations increased the viability of normal skin fibroblast primary cells.