12nd MBBS Student, KS Hegde Medical Academy, Nitte-Deemed to be University
2Research Scholar, KS Hegde Medical Academy, Nitte-Deemed to be University
3Professor, Department of Biochemistry, KS Hegde Medical Academy, Nitte-Deemed to be University
4Professor, Department of Medicine, KS Hegde Medical Academy, Nitte-Deemed to be University
*Corresponding Author E-mail: ushachidu@yahoo.com
Online published on 9 April, 2020.
Type 2 diabetes mellitus (T2DM) is a chronic disease associated with abnormal glucose metabolism due to insulin resistance. Leptin has been shown to be involved in pathways influencing the risk of cardiovascular disease and diabetes. The aim of the study was to evaluate pattern of leptin receptor gene polymorphism Gln223Arg in T2DM and to find its association with serum levels of leptin and also to compare leptin levels in diabetics and non-diabetics.
The cross-sectional study was conducted in Molecular Genetics division of KS Hegde Medical Academy, Mangalore. Genotyping of leptin receptor was done for Gln223Arg alleles by PCR-RFLP in 39 type 2 diabetics. Serum leptin was assayed using ELISA in 39 cases and 45 non-diabetic controls. Statistical analysis was carried out with Graph pad Instat version 3.
Hardy-Weinberg Equilibrium (HWE) for the LEPR gene variants showed that alleles were in equilibrium. Leptin levels were insignificantly low in diabetics compared to controls. Women in control groups showed significantly higher leptin levels (p<0.05) compared to men. There was no significant association between leptin levels among different genotypic variants.
We could conclude that type 2 diabetes mellitus is not associated with LepR gene polymorphism as well as there is independent relationship between LepR gene polymorphism and leptin levels
Nucleotide, Polymorphism, Diabetes Mellitus, Leptin, Receptor, Gene
