Potential activity of Madhuca longifolia leaf extract: through In vitro, Pharmacological and In silico studies

Madhuca longifolia is a universal panacea in the treatment as ayurvedic medicine which is traditionally valued for timber, flowers, fruits, leaves and seeds. The leaf of M.longifolia is known to have anti-diabetic, anti-oxidant, anti-ulcer and anti-inflammatory activities. The aim of our research is to know the beneficial activity of the leaf of M.longifolia through in vitro, pharmacological and in silico studies. The in vitro assays like Total phenolic content, DPPH assay, catalase activity and peroxidase activity was performed in ethanolic, methanolic and aqueous leaf extract of M.longifolia on serial dilution. The pharmacological activities were observed through the hot-plate method, analgesic method, antipyretic method and ulcerogenic method in female Wistar albino rats. The in silico analysis was performed through patch dock online server against the nuclear receptor and the active compounds of M.longifolia leaf. The 3-dimensional structures of the nuclear receptors were retrieved from RCBS protein data bank and the ligands were obtained from Corina molecular network. The in vitro assay demonstrated the antioxidant activity of all the leaf extract in which aqueous extract shown a potential antioxidant activity in 1: 2 serial dilutions. The aqueous leaf extract on rats at 500 mg/kg. b.w. p.o. dosage has M.longifolia beneficial pharmacological activities than other extract. The ligands like myricetin 3-O-L-rhamnoside, quercetin 3-galactoside, a-D-Mannopyranoside, D-Allose, myricetin, Quercetin, myricetin 3-O-arabinoside and 3′-Hydroxy- 4′-methoxydiclofenac has M.longifolia potential binding affinity with the receptors. Thus, the antioxidant, pharmacological and potential binding affinity of M.longifolia leaf was demonstrated in our research.