A Review on Comparative study of HPLC and UPLC

Recent advancements in pharmaceutical analysis have made available chromatographic media with a 1.7 μm particle size along with a liquid handling system that can operate such columns at much higher pressures. This technology, termed Ultra performance liquid chromatography (UPLC) using sub 2 micron particles and very high pressure (up to 100 MPa is possible in UPLC system) has demonstrated improvement in method sensitivity, resolution and speed compared with conventional HPLC. The UPLC system allows decreasing analysis time up to nine times compared to chromatographic system using 5 μm size particle packed analytical columns. In comparison with 3μm size particle packed analytical columns analysis time shortened about three times. The present review paper differentiates HPLC and UPLC, analytical method validation, applications, advantages and disadvantages of HPLC and UPLC.