Plant Mediated Synthesis, Characterization of Zinc Oxide Nanoparticles using Simarouba glauca and its Antibacterial Activities

Development of Nano biotechnology through green route is generating interest for researchers towards the production of eco-friendly and cost effective nanoparticles. In this regard, making use of floral parts for the nano particles synthesis has advantages such as cost effective, biocompatible and safe discharge. Using Simarouba glauca aqueous leaf extract, Zinc oxide nanoparticles (ZnO NPs) were synthesized from 1mM solution of Zinc nitrate. The presence of ZnO was confirmed at 340nm in Ultraviolet-visible (UV-Vis) spectroscopy and at 533-860cm-1 in Fourier transform infrared spectroscopic (FTIR) analysis. Further morphological phase identification was carried out by Transmission electron microscopy (TEM) and X-ray diffraction (XRD) analysis. Elemental confirmation and its quantitative measurement were performed by X-ray fluorescence spectroscopy (XRF) and energy-dispersive X-ray analysis spectroscopy (EDAX) Anti-bacterial activities exhibited inhibition against the bacterial strains Bacillus cereus CI 2106 (Gram-positive) and Klebsiella pneumonia ATCC 27736 (Gram-negative) at minimum concentrations.