An Apparatus for the determination of rat paw Edema during In vivo Evaluation of Anti-inflammatory agents

In vivo evaluation of anti-inflammatory agents normally involves the measurement of rat paw volume. Some notable equipment for in vivo measurement of rat paw volume includes plethysmometer, screw gauge and vernier calipers. Balance method is also reported for the purpose. The present study describes development and validation of a simple apparatus for the determination of rat paw volume suitable for in vivo evaluation of anti-inflammatory agents. The calibration plot obtained with the developed apparatus was linear with a high R2 value of 0.983. Thus it was confirmed that the apparatus is useful for the determination of rat paw volume.