1Department of Biology, Faculty of Science and Technology, Universitas Airlangga, 60115, Surabaya, Indonesia
2Animal Histology Laboratory, Faculty of Science and Technology, Universitas Airlangga, 60115, Surabaya, Indonesia
3Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, 60115, Surabaya, Indonesia
4Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, 60115, Surabaya, Indonesia
5Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia
6Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia
*Corresponding Author E-mail: dwi-w@fst.unair.ac.id
Online published on 4 July, 2020.
This study aims to evaluate the effects of alginate extract from Sargassum ilicifolium and okra (Abelmoschus esculentus) pods extract on the re-epithelialization rate of the wound area and the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density in streptozotocin-induced diabetic mice. This study was done in vivo using male mice (strain BALB/C, 3 to 4 months old, 30 to 40g). The normal control group (KN) and the diabetic groups (induced by streptozotocin). The diabetic group was divided into 9 groups: three diabetic control groups (KD), three alginate treatment groups (KAI), and three treatment groups of alginate-okra (Abelmoschus esculentus) pods extract (KAIO). The treatment dose was 50 mg/kg body weight. Measurement of fasting blood sugar levels was done before and after streptozotocin injection. The treatment was carried out in three different time periods: 3 days, 7 days, and 14 days. Then, mice skin was cut and proceed for histopathological analysis. Interestingly, we indicated that the administration of okra (Abelmoschus esculentus) pods extract combined with alginate from Sargassum ilicifolium could increase the rate of re-epithelialization of the wound area, increase the number of neutrophils, macrophages, fibroblasts, fibrocytes, as well as increase synthesized collagen. The combined extract was able to improve the healing process of open wounds in diabetic mice. Therefore, it can be concluded that the administration of alginate from Sargassum ilicifolium and okra (Abelmoschus esculentus) pods extract was able to improve open wounds healing in diabetic mice due to the role of antioxidant from polysaccharides and quercetin which acted as anti-hyperglycemic to anti-inflammatory properties.
Abelmoschus esculentus, Antioxidant, Diabetic Mice, Sargassum ilicifolium, Wound Healing
