An accurate and precise method developed and validated for Midazolam. Midazolam was analyzed by a Reverse phase high-performance liquid chromatography system with PDA detector (Agilent technologies 1220 Infinity II LC). LC solution software was used to record the data. C18- 250mm × 4.6mm and 5μm, using a mobile phase solution of ACN: water (70:30% v/v) mobile phase. Midazolam was detected at retention time 1.627 min using flow rate of 1.0ml/min at 220 nm. At 2–20μg/ml range, calibration was estimated with a regression coefficient of r2 is 0.997. Based on the recovery data, the current method was considered simple and accurate. Limit of Detection (LOD) were 0.17μg/ml and Limit of Quantification (LOQ) was 0.5μg/ml, values was found to be within the limits. This result shows that the method was well validated and quicker, cost-effective for routine analysis of Midazolam.
Development, Linearity, RP-HPLC, Validation, Midazolam
