Stability indicating hptlc method development and validation for the estimation of celecoxib in bulk drug and its pharmaceutical formulation

The current work is intended towards the development of a novel, simple and precise high-performance thin layer chromatography (HPTLC) method coupled with densitometer for estimation of celecoxib in bulk and its pharmaceutical formulated. The chromatographic development was performed on aluminum Plates coated with silica gel 60 F₂₅₄ using Toluene: Ethyl acetate: Methanol (8:2:1 v/v/v) as the mobile phase. Densitometric scanning was achieved at the absorbance maxima, at 254nm. Well separated band was observed with Rf value 0.63. The calibration curve was plotted in the concentration range from 100-600ng/spot exhibited an excellent linear relationship with R² value of 0.9997. The method was found to comply with all the validation parameters as per international conference on harmonization (ICH) guidelines. This method elaborates that the mobile phase which was used has minimal run time. This validated method can be used by quality control laboratories for the routine quantitative analysis of Celecoxib capsule.