1Asst Professor, Department of Pharmaceutical Analysis and Quality Assurance, Gland Institute of Pharmaceutical Science, Kothapet (V), Near Narsapur, Medak (District), Telangana-502334
2Asst Professor, Department of Pharmaceutics, Gland Institute of Pharmaceutical Science, Kothapet (V), Near Narsapur, Medak (District), Telangana-502334
3Asst Professor, Department of Pharmacognosy, Vishnu Institute of Pharmaceutical Education and Research, Narsapur (Mandal), Medak (District), Telangana-502334
4Asst Professor, Department of Pharmacology, Gland institute of Pharmaceutical Science, Kothapet (V), Near Narsapur, Medak (District), Telangana-502334
5Associate Professor, Department of Pharmaceutical Analysis and Quality Assurance, Raghavendra Institute of Pharmaceutical Education and Research, Krishnam Reddy Pally (V), Ananthapuramu-515721, Andhra Pradesh, India
*Corresponding Author E-mail: mohanbabu623@gmail.com
Online published on 16 September, 2020.
A simple, precise, accurate and economical UV-Spectrophotometric method has been developed and validated for the simultaneous estimation of Hydrocortisone and Iodoquinol in combined pharmaceutical dosage form using simultaneous equation method. Hydrocortisone has absorbance maxima at 245nm and Iodoquinol has absorbance maxima at 250nm in Methanol. The drugs obeyed beer's law in the concentration range of 10–60μg/ml for Hydrocortisone and 5–30μg/ml for Iodoquinol. The correlation coefficient of Hydrocortisone and Iodoquinol was found to be 0.999 and 0.999 respectively. The method was statistically validated as per the ICH guidelines. The low RSD values indicate good precision and high recovery values indicate accuracy of the proposed method. The developed method was simple, precise, accurate, reproducible and economical which can be efficiently and easily applied to pharmaceutical dosage form.
Hydrocortisone, Iodoquinol, UV-Spectroscopic method
