Synthesis, characterization and efficacy of antibiotic coated chitosan nanoparticles on human pathogens

The biodegradable nanoparticles of chitosan have received a significance attention as potential drug delivery vehicles in the recent decades. The present study was carried out to extract chitosan nanoparticles from shrimp shell waste and characterized followed by its potential antibacterial activity. Pure chitosan nanoparticles (CNP) and tetracycline coated nanoparticles (TCNP) were prepared by the ionotropic gelation process. The prepared nanoparticles were characterised by UV visible spectrophotometer, SEM, XRD, FTIR, NMR, Nano size - Zeta potential analysis. Antibacterial activity was assessed using CNP and TCNP against 7 different strains of human pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Salmonella typhi, S. paratyphi, Pseudomonas aeruginosa and Bacillus subtilis. In the present study, the CNP and TCNP had exhibited a growth inhibition activity on bacteria at lower concentration and proved as a remarkable drug carrier.