Homology modeling of microbial Nattokinase enzyme, An Anti-blood clotting (Fibrinolytic) agent using computational tools

Many people are suffering from cardiovascular diseases and some may die due severe heart strokes. The main reason for heart attacks is clots in the circulating blood due to fibrin. The Nattokinase enzyme which is produced by certain microbes especially Bacillus species is an effective drug for preventing cardiovascular disorders and other complications. The Nattokinase degrades fibrin and removes blood clots. The administration of Nattokinase had minimized the blood clots in patients. In the present paper the sequence of Nattokinase enzyme was derived from Uni Prot web-server and used to develop its three-dimensional model in Phyre2 server. The quality of developed model structure of Nattokinase enzyme was validated using the most widely used web programs Verify3D and ProSA. Further an improved Nattokinase enzyme can developed using various advanced bioinformatics tools. Such improved Nattokinase enzyme can be produced in the laboratory by microbes.