Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2020
  • Volume: 13
  • Issue: 9

Characterization of purified urinary human Follicle stimulating hormone

  • Author:
  • Sushil Yadaorao Raut1, Manjunath B Joshi2, Guruprasad Kalthur3, Srinivas Mutalik1,
  • Total Page Count: 5
  • Page Number: 4315 to 4319

1Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal, 576104, Karnataka State, India

2Department of Ageing Research, School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka State, India

3Department of Clinical Embryology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, Karnataka State, India

*Corresponding Author E-mail: ss.mutalik@manipal.edu

Online published on 16 September, 2020.

Abstract

Follicle stimulating hormone (FSH) is used widely for the treatment of female infertility. The present study reports various analytical tools to assess the physicochemical properties of FSH in order to investigate the structural integrity, purity and consistency of FSH.

FSH was characterized for appearance, UV absorption, circular dichroism (CD), molecular weight, FTIR as well as standard ELISA test.

Visually it is lyophilized, white colored amorphous powder. Histidine, aromatic amino acids,cysteine and methionine showed UV band at around 230nm. The UV absorption maxima value of FSH at the concentration of 5μg/mL in phosphate buffer saline pH 7.4 was found to be 212.4nm. The CD data revealed that pure FSH showed positive antiparallel β-pleated sheets at 195nm and negative antiparallel β-pleated sheets at 212nm, which is a well-defined conformation of the FSH secondary structures. SDS-PAGE showed bands with apparent molecular weight of ≍ 30kD. The FTIR analysis showed the characteristic peaks corresponding to carbonyl group (−C=O) of amide, peaks of secondary amide group (−CONH-) and amine (−NH-) group. Standard ELISA test clearly indicated the integrity of protein structure of FSH. The regression coefficient (R2) obtained for the linear equation was found to be 0.9998.

The present study revealed successful characterization of FSH.

Keywords

Follicle stimulating hormone, UV spectrum, Circular dichroism, ELISA