Validated uv-visible spectroscopic analytical method development and stability studies on oseltamivir

To develop and validate a simple accurate and precise UV-spectrophotometric method to determine the degradation pathway of Oseltamivir in bulk form.

Separation of the drug from its degradation product was achieved by UV and scanned between 200-400nm. Oseltamivir was subjected to stress condition such as acid hydrolysis, alkali hydrolysis, oxidation, photolysis and thermal degradation. The samples were analyzed.

The maximum absorbance was found at 216nm and found to be linear over the range of 5–30μg/ml with good correlation co-efficient 0.999. Major degradation was observed in photolysis, oxidative and alkali degradation.

The UV spectrophotometric method for Oseltamivir in the bulk form was developed and validated.