Combination effect of ciprofloxacin and streptomycin with cefotaxime against multi-drug resistant Pseudomonas aeruginosa from different clinical samples

To investigates the synergistic efficacy of synergistic antibiotic combinations effect on P. aeruginosa strains and evaluates the typical antimicrobial effects for Ciprofloxacin and Streptomycin with Cefotaxime on P. aeruginosa strains.

Pseudomonas Isolation Agar (PIA) has been employed to isolate a clinical P. aeruginosa. For minimum inhibitory concentration (MIC) of Cefotaxime, Ciprofloxacin, and Streptomycin, the combination antimicrobial effect of these antibiotics also determined based on half-chess board method. Standard strain of E. coli MM294 E. and E.coli HB101 has been used as control.

The clinical samples were resistant to Cefixime Ciprofloxacin by rates of 80%, 5%, respectively. Correspondingly, MICs of the Cefixime, Ciprofloxacin, and Streptomycin were between 32–1024μg/ml, 2–128μg/ml, 128–1224μg/ml respectively. The results of Ciprofloxacin-Streptomycin combination with Cefotaxime showed a synergic effect and a significant reduction in the MIC value at a ratio (1: 3) for 20 strains with percentage of 95.23%, while the other combinations have depicted different fallouts based on synergic, addition, indifference and Antagonism.

Cefotaxime with Streptomycin can weaken antibiotic resistance mechanisms of P.aeruginosa strains. It can make the Bacterium, as mentioned above, more susceptible to weaker concentrations of antibiotics.