UV Spectrophotometric Estimation of pioglitazone using multivariate method in bulk drug and pharmaceutical dosage form

Multivariate calibration technique makes the use for determination of Pioglitazone in bulk drug and pharmaceutical dosage form. This technique is constructed to manipulate statistical calculations, in correlating the relation between concentrations against absorbance at five separate wavelengths. λmax of the Pioglitazone was found to be 218nm. The results were statistically performed and offer ideal outcome by removing variations emerging from the instrumental or experimental conditions. The multivariate technique was approved according to ICH guidelines as well as originated to be sensitive, linear, accurate, precise, and consistent. The graph have been constructed to be straight-line over a concentration ranging from 8–16μg/mL with acceptance value of correlation coefficient (r2) of about 0.9969. Equation of linear regression has shown Y= 0.0486x + 0.1856 and both LOQ as well as LOD was originated to be 1.2765 and 3. 86831μg/mL individually. While %RSD for precision intraday as well as interday have been in range of 0.7343–0.9543 and 0.837–0.901 respectively. The percentage recovery was found within the range of 92.87–99.78% w/w. The results shows that the multivariate technique is simple, linear, precise, accurate, sensitivity and reproducible for the estimation of Pioglitazone in pharmaceutical dosage form.