Evaluation of Anti-inflammatory and Anti-diabetic activities of Actinodaphne madraspatana bedd leaves

The present study was carried out to find out the in-vivo anti-inflammatory and anti-diabetic activities of ethanol extract (200 and 400mg/kg) of leaves of Actinodaphne madraspatana (A. madraspatana) on Swiss Albino rats. The anti-inflammatory activity was investigated in carrageenan induced rat paw edema model which was compared with standard drug indomethacin at a dose level of 10mg/kg and the parameter measured being the paw volume by mercury displacements at 0, 15, 30, 60, and 120 minutes. The edema was induced in rats by administration of 1% w/v solution of carrageenan in normal saline solution (1%w/v). The anti-diabetic activity was investigated in streptozotocin induced diabetic rat, which was compared with standard drug glibenclamide at a dose level of 4 mg/kg and the parameter measured being the blood glucose level on 0, 7, 14, 21 days. Diabetes was induced in rats by administration of streptozotocin (60mg/kg) in ice cold citrate buffer (pH 4.3). Results of pharmacological activities revealed that the ethanol extract of the plant leaves showed the significant (p<0.001) anti-inflammatory and anti-diabetic activities in a dose of 200mg/kg and 400 mg/kg body weight. The ethanol extract of leaves of A.madraspatana possess the anti-inflammatory and anti-diabetic activities.