Development and Validation of spectrophotometric estimation of vildagliptin through Oxidative coupling reaction using MBTH reagent in Pharmaceutical dosage form

Scientifically estimation of Vildagliptin in bulk and pharmaceutical dosage forms by 3-methyl-2-bezothiazoline hydrazone reagent using longer wavelength give strong absorbance. The purpose of this analytical validation procedure is to validate using ICH guideline.3-methyl-2-bezothiazoline hydrazone reacts with ferric chloride in the presence of amine group, Resulting green coloured species scanned in uv-visible spectrophotometric with the range 400-800nm. Linearity concentration ranges10 to 50μg/ml and it given absorbance at 620nm, Correlation coefficient value 0.998, Recovery studies obtain within range of 98.41 to 99.75% at maximum absorbance 620nm. This method can be successfully applied for the estimation of drug content in pharmaceutical formulations. Resulting Analytical Validate method suitable for its intended use.