RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY

SCOPUS
  • Year: 2021
  • Volume: 14
  • Issue: 1

Effect of solvent on yield, phytochemicals and in vitro antioxidant potential of Rhododendron arboreum

  • Author:
  • Shefali Sharma1, Rupali Chaudhary1, Rajan Rolta1, Nitin Sharma2, Anuradha Sourirajan1, Kamal Dev1, Vikas Kumar1*
  • Total Page Count: 6
  • Page Number: 311 to 316

1Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, PO Sultanpur, Distt. Solan-173229 (HP), India.

2Department of Biotechnology, Chandigarh College of Technology, CGC Landran, 140307, Mohali, Punjab, India.

*Corresponding Author E-mail: vikaskmr59@gmail.com

Online published on 22 April, 2021.

Abstract

Rhododendron arboreum (family-Ericaceae) is one among the medicinally and economically important plant species from Himalayan region. Aim of present study was to study the effect of solvent on extraction yield, phytochemicals and antioxidant potential of various parts of R. arboretum. Extract of various solvents (n-hexane, methanol and water) were prepared using cold maceration method. Spectrophotometric method was used to quantify total phenolic and flavonoid content of different extracts. DPPH, FRAP and ABTS method was used to evaluate antioxidant activity of different extracts of various parts of R.arboreum. Among all solvent extract of different part of R.arboretum, highest extraction yield was observed in aqueous extract of leaf (10.78%) followed by methanolic extract of flowers (10.42%). Methanolic extract of flower showed higher amount of TPC (107.46±8.74 mg/g GAE) and TFC (254.26±29.33 mg/g RE). DPPH, FRAP and ABTS assay showed lowest IC50 value in flower extracts (methanolic and water) and hence validating the utilization of flower of this tree as source of natural antioxidants.

Keywords

Rhododendron arboretum, Cold maceration, Extraction yield, Antioxidant activity