Microbiological evaluation of clean rooms in pharmaceutical industries and molecular characterization of microbial isolates

Environmental monitoring in pharmaceutical industry was studied by using settle plate, air sampling, surface monitoring and personnel monitoring. These methods are commonly employed to collect the samples in pharmaceutical industry. The collected samples were incubated and predominant bacteria were isolated based on the colony morphology and identified by molecular characterisation. The PCR sequenced bi-directionally for seven isolates i.e, Micrococcus luteus, Bacillus sp, Bacillus Sp. Strain S3Sr84, Kocuria sp. LWYT2000(2016), Staphylococcus sp. Bca53(2016), Brevibacterium sanguinis strain CF52, Staphylococcus hominis strain HB14. Environmental monitoring statistical trend data will provide the process control and environmental isolate must be identified to understand their disinfectant susceptibility.