Cytotoxic and Antimicrobial Activities of Ethyl Acetate Extract from Fungus Trichoderma reesei strain JCM 2267, Aspergillus flavus strain MC-10-L, Penicillium sp, and Aspergillus fumigatus Associated with Marine Sponge Stylissa flabelliformis

This study was to investigate the cytotoxic and antimicrobial activities of ethyl acetate extract yielded from fermented fungi Trichoderma reesei strain: JCM 2267, Aspergillus flavus strain MC-10-L, Penicillium sp, and Aspergillus fumigatus associated with Stylissa flabelliformis sponge, and also to investigate the significant component in ethyl acetate extract of it. Antimicrobial experimentation was performed to various microbes with liquid microdilution method. The assessment of antimicrobials was undertaken by observing the value of MIC50. Cytotoxic testing was performed using MTT assay method. The cytotoxic activity evaluation was conducted by finding the value of IC50. The compound analysis in the ethyl acetate extract was used GC-MS. The result showed that in the antimicrobial experimentation, there was a variation in the percentage of inhibition where the smallest MIC50 values were found in Aspergillus fumigatus fungi ethyl acetate extract against Candida albicans ATCC 10231 at 1.3μg/mL. The result of the cytotoxic test against tumor cell line T47D, the lowest IC50 was found in Penicillium sp fungi with 111μg/mL value. Aspergillus flavus strain MC-10-L fungi, Penicillium sp, and Aspergillus fumigatus obtained from sponge isolation Stylissa flabelliformis have antimicrobial and cytotoxic activity with various range. All fungi extracts are not toxic against normal cells (Vero cells). The class of compounds in ethyl acetate fungi extract Trichoderma reesei strain JCM 2267 are mostly cyclohexane.