Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2021
  • Volume: 14
  • Issue: 10

UV Spectrophotometric Method Development and Validation of Lignocaine Hydrochloride in Bulk and Semisolid Dosage Form

  • Author:
  • Suyash Ingle, Varsha Tegeli, Avinash Birajdar, Vinod Matole, Sagar Adlinge, Gajanand Nangare
  • Total Page Count: 3
  • Page Number: 5280 to 5282

D.S.T.S Mandal's College of Pharmacy, Solapur - 413004, Maharashtra, India

*Corresponding Author E-mail: suyashingle18@gmail.com

Online Published on 22 February, 2022.

Abstract

A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation.

The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer's law is valid in the concentration range of 20–100μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness.

The method has demonstrated excellent linearity over the range of 20–100μg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml).

Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.

Keywords

Lignocaine Hydrochloride, UV spectroscopy, Method development and validation, RO Water, Semisolid Formulation