D.S.T.S Mandal's College of Pharmacy, Solapur - 413004, Maharashtra, India
*Corresponding Author E-mail: suyashingle18@gmail.com
Online Published on 22 February, 2022.
A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation.
The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer's law is valid in the concentration range of 20–100μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness.
The method has demonstrated excellent linearity over the range of 20–100μg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml).
Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.
Lignocaine Hydrochloride, UV spectroscopy, Method development and validation, RO Water, Semisolid Formulation
